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Applied Physics Letters / Volume 99 / Issue 26 / BIOPHYSICS AND BIO-INSPIRED SYSTEMS

Appl. Phys. Lett. 99, 263703 (2011); http://dx.doi.org/10.1063/1.3672225 (3 pages)

Direct observation of dynamic force propagation between focal adhesions of cells on microposts by atomic force microscopy

Akinori Okada, Yusuke Mizutani, Agus Subagyo, Hirotaka Hosoi, Motonori Nakamura, Kazuhisa Sueoka, Koichi Kawahara, and Takaharu Okajima

Graduate School of Information Science and Technology, Hokkaido University, Kita-ku N14 W9, Sapporo 060-0814, Japan

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(Received 3 September 2011; accepted 29 November 2011; published online 29 December 2011)

We investigated dynamic force propagation between focal adhesions of fibroblast cells cultured on polydimethylsiloxane micropost substrates, by atomic force microscopy. Live cells were mechanically modulated by the atomic force microscopy probe bound to cell apical surfaces at 0.01–0.5 Hz, while microposts served as a force sensor at basal surfaces. We observed that cells exhibited rheological behavior at the apical surface but had no apparent out-of-phase response at the basal surface, indicating that the dynamic force propagating through cytoskeletal filaments behaves in an elastic manner. Moreover, the direction of the propagated force was observed to be intimately associated with the prestress.

© 2011 American Institute of Physics

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KEYWORDS and PACS

Keywords

adhesion, atomic force microscopy, biological techniques, biomechanics, cellular biophysics

PACS

  • 87.17.Rt

    Cell adhesion and cell mechanics

  • 87.64.Dz

    Scanning tunneling and atomic force microscopy

  • 87.80.Ek

    Mechanical and micromechanical techniques

ARTICLE DATA

Digital Object Identifier
http://dx.doi.org/10.1063/1.3672225

PUBLICATION DATA

ISSN

0003-6951 (print)  
1077-3118 (online)

Publisher

$abstract.society.value is a Member of CrossRef American Institute of Physics

For access to fully linked references, you need to log in.
    B. Fabry, G. N. Maksym, J. P. Butler, M. Glogauer, D. Navajas, and J. J. Fredberg, Phys. Rev. Lett. 87, 148102 (2001).


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